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Then cells were washed three times with PBS followed by incubation with the secondary antibody (1:200, Proteintech) and Hoechst 33342 (1:1000) for 1 h
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Cell dysmetabolism has also been the subject of recent reviews that explore its association with the pathogenesis of neurodegenerative diseases such as prion disease, Alzheimerβs, and Parkinsonβs disease
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