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In contrast, expression of hsp-4 , a canonical ER-stress marker, and CPL-1* ( nhx-2::cpl-1W32A,Y35A::yfp ), an ER-associated degradation substrate 58,59 , was not induced by rnp-6 mutation (Supplementary Fig
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The optical density was immediately measured using a multifunction microplate reader (Tecan) at 593 nm
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