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For mild deficiency in people who absorb nutrients normally, high-dose oral B12 can be equally effective

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The use of additives should be carefully considered as they might compromise the target protein structure and activity ( Proteins bound to HIC media can be eluted using some of the above-mentioned conditions such as reduced salt concentration, increased pH, or addition of alcohols or detergents (Lienqueo et al., 2007), but trial-and-error experiments should be conducted to select the best option for each specific target protein