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In general, the amino heterocycle strongly contributes to binding, in fact, the positively charged amino group forms a salt bridge with Glu467 (N-O distance: 2.7 Å for piperazine, 3.6 Å for piperidine), while the other main interactions are hydrophobic and involve Ile339, Phe396, Leu399 (Figure 4B)

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[7] (This interaction is not significant when levodopa is combined with carbidopa, as in standard carbidopa/levodopa therapy, because carbidopa prevents peripheral levodopa breakdown.) Another notable interaction involves the chemotherapeutic agent altretamine: coadministration of pyridoxine can decrease altretamine’s effectiveness and is generally advised against unless specifically directed