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As reviewed by Hitchler et al., the intracellular redox chemistry has the potential to generate significant alterations in the epigenetic landscape through GSH and iron ions, which curtail the accessibility of epigenetic co-factors including S -adenosylmethionine (SAM), α-ketoglutarate, ascorbate, and nicotine adenine dinucleotide (NAD + ) (Hitchler and Domann, 2021)

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For a 150 min run, peptides were eluted over a 150 min gradient using 80% acetonitrile, and 0.1% formic acid (buffer B), going from 2% to 8% over 10 min, to 45% over 115 min, then to 65% over 20 min, and then kept at 95% for 10 min, after which all peptides were eluted

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