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AAV production for Chac2 silencing To construct AAV vectors for the specific silencing of Chac2 in mouse BM neutrophils, we first selected target sequences for mouse Chac2 mRNA using the application program from Dharmacon siDESIGN center ( The specific shRNA sequence 5’-CTACAGAACTACGACAGTCAT-3’ was designed
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This is facilitated by IOP1 (iron-only hydrogenase-like protein) and the CIA-targeting complex [57]
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