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Increased cytoplasmic calcium ion concentration, disrupted mitochondrial membrane potential, and increased oxidative stress after the treatment of silibinin in VK2/E6E7 and End1/E6E7 cells were observed.258 Oxidative stress, and disturbance of endoplasmic reticulum calcium metabolism may lead to elevated expression of endoplasmic reticulum stress-related proteins such as IRE1, ATF6, and PERK, which are responsible for ERS and signal transmission
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A thioredoxin gene fusion expression system that circumvents inclusion body formation in the Escherichia coli cytoplasm
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