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Quantitative analysis of CEdG immunofluorescence metrics To determine the quantitative abundance of CEdG in OSCC and NO nuclei, nuclear and cytoplasmic metrics such as nuclei count, mean fluorescence intensity (MFI), nuclear MFI (N-MFI), cytoplasmic MFI (C-MFI), the nuclear-to-cytoplasmic MFI ratio (N/C-MFI), coefficient of variation (CV), and H-score were quantified using imageJ software

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Step five: the escalation schedule Once you have established your starting dose, follow a 4-week escalation pattern