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In brief, separation was achieved on an Agilent Eclipse Plus C18 150 × 2.1 mm 1.8 um column with mobile phases of water with 0.1% acetic acid as mobile phase A and acetonitrile/methanol (84/16) with 0.1% acetic acid as mobile phase B
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It mimics the action of ghrelin, the endogenous hunger hormone, triggering GH release via IP3/DAG second messenger pathways rather than the cAMP pathway used by GHRH
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