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All the vectors were verified by sequencing, and detailed information on these plasmids is available upon request ( Generation of Expression Lentivirus The lentivirus for human ERβ and SOD2 was prepared as described previously in our lab ( ctcgag - atg tcc atc tgt gcc tct tct -3′ (Xho1) and mouse ERβ reverse primer: 5′- gtac- tctaga - tca ctg tga ctg gag gtt ctg -3′ (Xba1)

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Oral doses in published rodent studies tend to be set at roughly 10 mcg/kg, which is at the higher end compared to injectable doses in the same literature