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Cell culture and peptide handling The HCC line HepG2 (American Type Culture Collection (ATCC) Manassas, VA, USA) was maintained in Dulbecco’s modified Eagle’s media (DMEM) with 4.5 g/l glucose (Corning, Corning, NY), 10% fetal bovine serum (Corning), and 100 U/ml penicillin and streptomycin (Gibco)
Growth Hormone Research
Quantity::10 MG
In addition, it has a drug-affinity complex (DAC) attached, enhancing its half-life to up to 8 days [25, 26]
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