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As for quantification of on-target editing efficiency, RAW264.7 macrophages were stimulated with LPS and treated with AO 12 LNPs loaded with Cas9 mRNA and NLRP3 sgRNA
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Mechanistically, the TCN2 variant acts on the carrier protein, downstream of absorption but upstream of cellular processing โ a step that is form-agnostic, since transcobalamin binds cobalamin regardless of its original axial group
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